Actalent
*Description:*
Minimum Qualifications:
A master’s degree with 5+ years of experience in cell biology. Preferred Qualifications:
PhD with 2+ years of experience in cell biology Position Objective Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, developing gene editing strategies, and developing assays to monitor editing. Scope of Need The successful individual will directly interact with senior R&D scientists and project management to execute project work plans. The scientist will be responsible for supporting or executing these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team. This role will involve the following tasks:
Mammalian cell culture
DNA delivery and mammalian cell transfection
Generation of Lentivirus
Genomic editing in mammalian cell lines (TALN and CRISPR based editing, design and implementation)
Transposase
Flow cytometric analysis
Molecular biology o Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR This individual will also be expected to support and participate in R&D and project management meetings. Capabilities and Competencies This position requires the following capabilities and competencies: o Proven experience in cell biology o mammalian cell culture o Lipid-based cell transfection and electroporation o Cell engineering (CRISPR or TALEN) o DNA transposase o Stable cell line generation o Cell-based assays, such as FACS, immunocytochemistry, ELISA, and Western Blotting o Experience in DNA transposition o Sleeping beauty o PiggyBac o CRISPR-guided transposase o Experience in molecular biology o DNA cloning and sequencing o DNA, RNA preparation o PCR, qPCR assays o Ability to: o Observe deviations from the expected outcomes and trouble-shoot o Succeed with a moderate level of supervision and guidance o Participate in a strong teamwork orientation with demonstrable influence/interpersonal skills o Maintain a detailed lab notebook and present your findings in a group setting Contacts: The Program Manager will interact with internal employees and management, as well as third-party providers, and customers. Working Conditions: The vast majority of working time will be spent in a laboratory environment. Cell work will take place in biosafety hoods in a tissue culture laboratory, working with various cell cultures. Time may be spent manipulating DNA constructs and proteins in a laboratory environment. Some time will be spent at the desk recording laboratory activities appropriately in notebooks. Candidate must be capable of observing and adhering to lab safety standards and protocols. This individual must be able to identify deviations from expected outcomes and participate in troubleshooting those deviations and suggesting alternatives. Critical to success in this position will be the individuals ability to work independently on some projects and to provide support collaboratively on others while meeting project timelines. Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator). Minimum Qualifications:
A masters degree with 5+ years of experience in cell biology. Preferred Qualifications:
PhD with 2+ years of experience in cell biology
Skills:*
cell engineering, mammalian cell culture, cell based assay, DNA transposition, cell biology, Stable cell line generation, dna preparation, crispr, Talen, lipid-based cell transfection, FACS, immunocytochemistry, elisa, western blot, Sleeping beauty, PiggyBac, dna cloning, dna sequencing, RNA preparation, PCR, qPCR, Genomic Editing, Flow Cytometric analysis
Top Skills Details:*
cell engineering,mammalian cell culture,cell based assay,DNA transposition,cell biology,Stable cell line generation
Additional Skills & Qualifications:*
Mammalian cell culture
DNA delivery and mammalian cell transfection
Generation of Lentivirus
Genomic editing in mammalian cell lines (TALN and CRISPR based editing, design and implementation)
Transposase
Flow cytometric analysis
Molecular biology o Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR
Experience Level:*
Entry Level
About Actalent:
Actalent connects passion with purpose. Our scalable talent solutions and services capabilities drive value and results and provide the expertise to help our customers achieve more. Every day, our experts around the globe are making an impact. We’re supporting critical initiatives in engineering and sciences that advance how companies serve the world. Actalent promotes consultant care and engagement through experiences that enable continuous development. Our people are the difference. Actalent is an operating company of Allegis Group, the global leader in talent solutions. The company is an equal opportunity employer and will consider all applications without regards to race, sex, age, color, religion, national origin, veteran status, disability, sexual orientation, gender identity, genetic information or any characteristic protected by law.
To apply for this job please visit careers.actalentservices.com.